Coding

Part:BBa_K874001:Design

Designed by: Ernst Bank   Group: iGEM12_Amsterdam   (2012-09-19)

Polydactyl Zinc Finger (PZF3838)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the PZF does not contains any forbidden/undesirable specified by the [http://dspace.mit.edu/bitstream/handle/1721.1/45138/BBFRFC10.txt RFC-10] protocol [1].
Since the individual Zinc Fingers in this PZF (or any PZF for that matter) are highly similar in sequence we strongly recommender that when doing PCR primers are used that ad-heal outside of the actual PZF.

Source

Created by Bert van der Zaal, Paul Hooykaas and Sylvia de Pater for use in their research on Arabidopsis.[2] But kindly provided for use in the iGEM Amstedam 2012 project.

References

  1. Knight, T. (2007). [http://dspace.mit.edu/handle/1721.1/45138 Draft Standard for Biobrick Biological Parts]. OpenWetWare
  2. de Pater S, Neuteboom LW, Pinas JE, Hooykaas PJ, van der Zaal BJ. (2009). [http://www.ncbi.nlm.nih.gov/pubmed/19754840 ZFN-induced mutagenesis and gene-targeting in Arabidopsis through Agrobacterium-mediated floral dip transformation]. Plant Biotechnol J.